Summary:
FWA 2 is bridge writing assignment to allow you update your scientific paper with feedbacks received from FWA 1 and complete your methods section. This assignment is due in class Tuesday, March 28 after showing your work to your instructor. To get full point be sure to include the following:
Title: Your title for the BEAF project must include the model’s name (organism and protein) and research question. Here’re a couple of starter phrases to guide you with crafting your own title: Finding physical interaction between Drosophila chromatin insulator protein BEAF and …….. ; What are the interacting partners of Drosophila chromatin insulator protein BEAF?
Introduction: A synopsis of what to include in your introduction is given below. This part of your scientific paper allows you to provide background information for your readers. The background MUST be relevant to the project and should flow from broad, general topics, to narrower topics and more specific ones. You MUST include *in-text citations (in APA format). At least your FWA should follow the suggested format below (Note: do not include the title of each paragraph in your introduction):
Paragraph 1: Gene expression and chromatin structure
Paragraph 2: Chromatin insulators
Paragraph 3: BEAF and your assigned proteins
Paragraph 4: Research question: Does BEAF physically interact with any of the experimental proteins (row, dref, rib, wds, dre4, ssrp, map60, apt)? Looking for pairwise interaction between BEAF and any of the proteins
Paragraph 5:
Methods: Briefly describe the methods you would use to answer the research question: Here’s a suggested starter phrase: In order to explore the interaction between BEAF and row (and include your assigned protein), we cloned BEAF into Gal4 DNA binding domain plasmid (pOBD) and row (and your assigned protein) into Gal4 activation domain plasmid (pOAD). These plasmids were tested for physical interaction in yeast cells using the yeast-two hybrid assay.
Hypothesis: Using the yeast two-hybrid assay, there will be physical interaction between BEAF and apt (an example)
Prediction: BEAF will not physically interact with apt (You give prediction based on available literatures, then you support it with in-text citations)
Methods: Use the Methods Complete document on Moodle. Copy and paste all the methods from the document into your FWA.
References: Provide a complete list of references used. You should have 5 or more references (that you already cited in your introduction). These references MUST be APA-formatted. You can get APA-formatted references from Google Scholar: Under your paper of interest, click the “Cite” option to view the citations for the paper. from there copy the APA-formatted reference.
* Remember the following:
In-text citations: You should include in-text citations in the body of your write up EVEN if you rephrase or paraphrase them. They are written after the statement BEFORE full stop.
In-text citations have author names and year:
two-authors example: (McDonald and Tom, 2019)
one-author example: (McDonald, 2019)
more than one author example: (McDonald et al., 2019)Summary:
FWA 2 is bridge writing assignment to allow you update your scientific paper with feedbacks received from FWA 1 and complete your methods section. This assignment is due in class Tuesday, March 28 after showing your work to your instructor. To get full point be sure to include the following:
Title: Your title for the BEAF project must include the model’s name (organism and protein) and research question. Here’re a couple of starter phrases to guide you with crafting your own title: Finding physical interaction between Drosophila chromatin insulator protein BEAF and …….. ; What are the interacting partners of Drosophila chromatin insulator protein BEAF?
Introduction: A synopsis of what to include in your introduction is given below. This part of your scientific paper allows you to provide background information for your readers. The background MUST be relevant to the project and should flow from broad, general topics, to narrower topics and more specific ones. You MUST include *in-text citations (in APA format). At least your FWA should follow the suggested format below (Note: do not include the title of each paragraph in your introduction):
Paragraph 1: Gene expression and chromatin structure
Paragraph 2: Chromatin insulators
Paragraph 3: BEAF and your assigned proteins
Paragraph 4: Research question: Does BEAF physically interact with any of the experimental proteins (row, dref, rib, wds, dre4, ssrp, map60, apt)? Looking for pairwise interaction between BEAF and any of the proteins
Paragraph 5:
Methods: Briefly describe the methods you would use to answer the research question: Here’s a suggested starter phrase: In order to explore the interaction between BEAF and row (and include your assigned protein), we cloned BEAF into Gal4 DNA binding domain plasmid (pOBD) and row (and your assigned protein) into Gal4 activation domain plasmid (pOAD). These plasmids were tested for physical interaction in yeast cells using the yeast-two hybrid assay.
Hypothesis: Using the yeast two-hybrid assay, there will be physical interaction between BEAF and apt (an example)
Prediction: BEAF will not physically interact with apt (You give prediction based on available literatures, then you support it with in-text citations)
Methods: Use the Methods Complete document on Moodle. Copy and paste all the methods from the document into your FWA.
References: Provide a complete list of references used. You should have 5 or more references (that you already cited in your introduction). These references MUST be APA-formatted. You can get APA-formatted references from Google Scholar: Under your paper of interest, click the “Cite” option to view the citations for the paper. from there copy the APA-formatted reference.
* Remember the following:
In-text citations: You should include in-text citations in the body of your write up EVEN if you rephrase or paraphrase them. They are written after the statement BEFORE full stop.
In-text citations have author names and year:
two-authors example: (McDonald and Tom, 2019)
one-author example: (McDonald, 2019)
more than one author example: (McDonald et al., 2019)