CRISPR/Cas9 Mutagenesis Reveals Versatile Roles of Hox Genes in Crustacean Limb Specification and Evolution

i will attach the source the i am allowed to use, and i will attach a 

second link to the rest of the method because they include diffrent infromation there. so please use both of them. please read the 

method part and analysis and discussion and address there 
approch. 
Approach/Method. What method is being used to generate mutant
lines/organisms? Briefly highlight some basics of that method.
Provide a simple flow chart to highlight how this screen was conducted.
link to artical: 
https://osu.instructure.com/courses/135155/files/52921729/download?download_frd=1https://osu.instructure.com/courses/135155/files/52921729/download?download_frd=1
the second link for rest of method: 
https://ars.els-cdn.com/content/image/1-s2.0-S0960982215014153-mmc1.pdfhttps://ars.els-cdn.com/content/image/1-s2.0-S0960982215014153-mmc1.pdf

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